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il 8  (R&D Systems)


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    R&D Systems il 8
    Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, <t>IL-8,</t> and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.
    Il 8, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dy208/Human+IL-8%2FCXCL8+DuoSet+ELISA/pmc13054578-81-31-33
    Average 96 stars, based on 633 article reviews
    il 8 - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis"

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    Journal: Military Medical Research

    doi: 10.1016/j.mmr.2026.100010

    Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, IL-8, and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.
    Figure Legend Snippet: Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, IL-8, and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Techniques Used: Concentration Assay, Incubation, Ex Vivo, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Bacteria

    Diagnostic performance for the detection of bacteremia, analyzing the neutrophil phenotype by determining the median fluorescence intensity (MFI) and the cellular response capacity (CRC) in comparison with traditional markers of humoral inflammation (IL-6, IL-8, MMP9). a Comparison of receiver operating characteristic (ROC) at 10,000 CFU/ml Escherichia coli ( E. coli ) with the respective 95% confidence interval (CI) and P -value, and half-maximal effective concentration (EC 50 ) as a function of the E. coli concentration. b Detailed comparison of the EC 50 as a function of the E. coli concentration. c Exemplary comparison of EC 50 curve fit after normalization as indicated by EC 50 (%) on the respective Y-axis to BuC=100% and 50 000 CFU/ml E. coli =0% for the humoral marker IL-6 (the IL-6 values were multiplied by −1 before EC 50 calculation to facilitate comparability with the CRC) and the change in neutrophil phenotype represented by CD11b CRC. BuC indicates buffer control after 60 min incubation; numbers on the X-axis of c indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, evaluating the EC 50 of IL-8, MMP9, the MFI, and CRC of CD10, CD11b, and CD62L in comparison to the EC 50 of IL-6. P -values are indicated above the respective data points. ⁎ P <0.05. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.
    Figure Legend Snippet: Diagnostic performance for the detection of bacteremia, analyzing the neutrophil phenotype by determining the median fluorescence intensity (MFI) and the cellular response capacity (CRC) in comparison with traditional markers of humoral inflammation (IL-6, IL-8, MMP9). a Comparison of receiver operating characteristic (ROC) at 10,000 CFU/ml Escherichia coli ( E. coli ) with the respective 95% confidence interval (CI) and P -value, and half-maximal effective concentration (EC 50 ) as a function of the E. coli concentration. b Detailed comparison of the EC 50 as a function of the E. coli concentration. c Exemplary comparison of EC 50 curve fit after normalization as indicated by EC 50 (%) on the respective Y-axis to BuC=100% and 50 000 CFU/ml E. coli =0% for the humoral marker IL-6 (the IL-6 values were multiplied by −1 before EC 50 calculation to facilitate comparability with the CRC) and the change in neutrophil phenotype represented by CD11b CRC. BuC indicates buffer control after 60 min incubation; numbers on the X-axis of c indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, evaluating the EC 50 of IL-8, MMP9, the MFI, and CRC of CD10, CD11b, and CD62L in comparison to the EC 50 of IL-6. P -values are indicated above the respective data points. ⁎ P <0.05. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Techniques Used: Diagnostic Assay, Fluorescence, Comparison, Concentration Assay, Marker, Control, Incubation, Bacteria

    Clinical specifications and parameters over all time points of the sepsis cohort. a Suspected infection cause of sepsis. b Distribution of the individual score points of the Sequential Organ Failure Assessment (SOFA) score. c Total SOFA score. d-h Traditional and humoral markers of inflammation: leukocytes and neutrophil-lymphocyte ratio ( d ), C-reactive protein (CRP) and procalcitonin (PCT) ( e ), interleukin-6 (IL-6) and interleukin-8 (IL-8) ( f ), serum amyloid A (SAA) and calprotectin ( g ), matrix metallopeptidase 9 (MMP9) and myeloperoxidase (MPO) ( h ). Values are shown as median and interquartile range. n =14. CNS. Central nervous system; HV. Healthy volunteers.
    Figure Legend Snippet: Clinical specifications and parameters over all time points of the sepsis cohort. a Suspected infection cause of sepsis. b Distribution of the individual score points of the Sequential Organ Failure Assessment (SOFA) score. c Total SOFA score. d-h Traditional and humoral markers of inflammation: leukocytes and neutrophil-lymphocyte ratio ( d ), C-reactive protein (CRP) and procalcitonin (PCT) ( e ), interleukin-6 (IL-6) and interleukin-8 (IL-8) ( f ), serum amyloid A (SAA) and calprotectin ( g ), matrix metallopeptidase 9 (MMP9) and myeloperoxidase (MPO) ( h ). Values are shown as median and interquartile range. n =14. CNS. Central nervous system; HV. Healthy volunteers.

    Techniques Used: Infection

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    Staining:

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    SYBR Green Assay:

    Article Title: Staphylococcus epidermidis activates keratinocyte cytokine expression and promotes skin inflammation through the production of phenol-soluble modulins.
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    Real-time Polymerase Chain Reaction:

    Article Title: Staphylococcus epidermidis activates keratinocyte cytokine expression and promotes skin inflammation through the production of phenol-soluble modulins.
    Article Snippet: 1457 Dpsmε (1B 1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 Dhld (1C-1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DpsmdDhld (D5-1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DecpA (1C-1(1)) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DpsmdDhldDecpA (1D-2(1)) Gallo (UCSD) This Study E. coli Chemically Competent One Shot Top 10 Invitrogen Cat#C404010 E. coli (DC10B) Monk and Foster18 N/A Chemicals, peptides, and recombinant proteins 2-mercaptoethanol Sigma-Aldrich Cat# M6250 Antibiotic-Antimycotic (1003) Gibco Cat# 15240062 Bacto Agar BD Biosciences Cat# 214010 Calcium chloride dihydrate Sigma-Aldrich Cat# 22,350-6 Defined Trypsin Inhibitor (DTI) Gibco Cat# R-007-100 DPBS Gibco Cat# 14190-144 Human keratinocyte growth supplement (HKGS) Gibco Cat# S0015 EpiLife complete medium, with 60 M calcium Gibco Cat# MEPI500CA RPMI medium Gibco Cat#11875093 Liberase TL Roche Cat# 5401020001 DNAse Sigma Cat#D5025 Fetal Bovine Serum Corning Cat#35010CV Ethyl alcohol, Pure Sigma-Aldrich Cat# E7023 Formalin Azer Scientific Cat# PFNBF-20 Lysing Matrix B MP Biomedical Cat# 116911050-CF Lysing Matrix D MP Biomedical Cat# 116913050-CF Molecular biology grade TE buffer Invitrogen Cat# AM9849 (Continued on next page) 12 Cell Reports 42, 113024, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAlater Stabilization Solution Invitrogen Cat# AM7021 Tryptic soy Broth (TSB) Sigma-Aldrich Cat# T8907-1KG Trypsin/EDTA solution Gibco Cat# R-001-100 UltraPure distilled water Invitrogen Cat# 10977-015 Q5 Hot Start High Fidelity Polymerase Master Mix (2x) NEB Cat#M0494L Ultrapure Agarose Thermofisher Scientific Cat# 16500500 1kb Plus DNA Ladder NEB Cat#N3200L Sybr Safe DNA Gel Stain Thermofisher Scientific Cat#S33102 SYBR Green qPCR Master Mix (23) Biomiga Cat# QP1311-02 Prolong Antifade Gold Thermofisher Scientific Cat# P36934 S. epidermidis PSMd, fMSIVSTIIEVVKTIVDIVKKFKK Lifetein N/A S. epidermidis PSMa, fMADVIAKIVEIVKGLIDQFTQK Lifetein N/A S. epidermidis PSMe, fMFIINL VKKVISFIKGLFGNNENE Lifetein N/A S. epidermidis d-toxin (hld), fMAADIISTIGDLVKWIIDTVNKFKK Lifetein N/A Optima LC/MS Water Fisher Scientific CAS 7732-18-5 Optima LC/MS Acetonitrile Fisher Scientific CAS 75-05-8 Optima LC/MS Formic Acid Fisher Scientific CAS 64-18-6 Optima LC/M Methanol Fisher Scientific CAS 67-56-1 Critical commercial assays iSCRIPT cDNA synthesis Kit BIO-RAD Cat#1708891 PureLink RNA Mini Kit Invitrogen Cat# 12183025 PureLink Microbiome DNA Purification Kit Invitrogen Cat# A29790 Human IL-8/CXCL8 DuoSet ELISA R&D Systems Cat# DY208 Deposited data Human keratinocyte S. epidermidis PSM treatment RNA-seq (bulk) This Study GEO: GSE209653 Murine S. epidermidis PSM treatment RNA-seq (bulk) This Study GEO: GSE210046 Experimental models: Cell lines Human Epidermal Keratinocytes, neonatal (HEKn) Gibco Cat# C0015C Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Strain: 000664 Oligonucleotides S. epidermidis psmd 1kb Upstream Forward (KpnI) 50-GTCCGGTACCCACAA ATAATGTTGCACCCC-30 This Study S. epidermidis psmd 1kb Upstream Reverse (SacII) 50-GTTCCCGCGGGTTCAT GACCTCCTTTCAAAAGG-30 This Study S. epidermidis psmd 1kb Down Forward (SalI) 50- GTCCGTCGACCGCTG AGAGTAATCATTAATTTGC-30 This Study S. epidermidis psmd 1kb Down Reverse (SacII) 50- GTCACCGCGGTTCTA CATGGGCCTGG-30 This Study S. epidermidis psmd 1kb Internal Forward 50-AGCAAGAGTGTCAA TGGTTAC-30 This Study S. epidermidis psmd 1kb Internal Reverse 50-CTAAGAAAGCGAGC CAAC-30 This Study (Continued on next page) Cell Reports 42, 113024, September 26, 2023 13 ..

    cDNA Synthesis:

    Article Title: Staphylococcus epidermidis activates keratinocyte cytokine expression and promotes skin inflammation through the production of phenol-soluble modulins.
    Article Snippet: 1457 Dpsmε (1B 1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 Dhld (1C-1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DpsmdDhld (D5-1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DecpA (1C-1(1)) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DpsmdDhldDecpA (1D-2(1)) Gallo (UCSD) This Study E. coli Chemically Competent One Shot Top 10 Invitrogen Cat#C404010 E. coli (DC10B) Monk and Foster18 N/A Chemicals, peptides, and recombinant proteins 2-mercaptoethanol Sigma-Aldrich Cat# M6250 Antibiotic-Antimycotic (1003) Gibco Cat# 15240062 Bacto Agar BD Biosciences Cat# 214010 Calcium chloride dihydrate Sigma-Aldrich Cat# 22,350-6 Defined Trypsin Inhibitor (DTI) Gibco Cat# R-007-100 DPBS Gibco Cat# 14190-144 Human keratinocyte growth supplement (HKGS) Gibco Cat# S0015 EpiLife complete medium, with 60 M calcium Gibco Cat# MEPI500CA RPMI medium Gibco Cat#11875093 Liberase TL Roche Cat# 5401020001 DNAse Sigma Cat#D5025 Fetal Bovine Serum Corning Cat#35010CV Ethyl alcohol, Pure Sigma-Aldrich Cat# E7023 Formalin Azer Scientific Cat# PFNBF-20 Lysing Matrix B MP Biomedical Cat# 116911050-CF Lysing Matrix D MP Biomedical Cat# 116913050-CF Molecular biology grade TE buffer Invitrogen Cat# AM9849 (Continued on next page) 12 Cell Reports 42, 113024, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAlater Stabilization Solution Invitrogen Cat# AM7021 Tryptic soy Broth (TSB) Sigma-Aldrich Cat# T8907-1KG Trypsin/EDTA solution Gibco Cat# R-001-100 UltraPure distilled water Invitrogen Cat# 10977-015 Q5 Hot Start High Fidelity Polymerase Master Mix (2x) NEB Cat#M0494L Ultrapure Agarose Thermofisher Scientific Cat# 16500500 1kb Plus DNA Ladder NEB Cat#N3200L Sybr Safe DNA Gel Stain Thermofisher Scientific Cat#S33102 SYBR Green qPCR Master Mix (23) Biomiga Cat# QP1311-02 Prolong Antifade Gold Thermofisher Scientific Cat# P36934 S. epidermidis PSMd, fMSIVSTIIEVVKTIVDIVKKFKK Lifetein N/A S. epidermidis PSMa, fMADVIAKIVEIVKGLIDQFTQK Lifetein N/A S. epidermidis PSMe, fMFIINL VKKVISFIKGLFGNNENE Lifetein N/A S. epidermidis d-toxin (hld), fMAADIISTIGDLVKWIIDTVNKFKK Lifetein N/A Optima LC/MS Water Fisher Scientific CAS 7732-18-5 Optima LC/MS Acetonitrile Fisher Scientific CAS 75-05-8 Optima LC/MS Formic Acid Fisher Scientific CAS 64-18-6 Optima LC/M Methanol Fisher Scientific CAS 67-56-1 Critical commercial assays iSCRIPT cDNA synthesis Kit BIO-RAD Cat#1708891 PureLink RNA Mini Kit Invitrogen Cat# 12183025 PureLink Microbiome DNA Purification Kit Invitrogen Cat# A29790 Human IL-8/CXCL8 DuoSet ELISA R&D Systems Cat# DY208 Deposited data Human keratinocyte S. epidermidis PSM treatment RNA-seq (bulk) This Study GEO: GSE209653 Murine S. epidermidis PSM treatment RNA-seq (bulk) This Study GEO: GSE210046 Experimental models: Cell lines Human Epidermal Keratinocytes, neonatal (HEKn) Gibco Cat# C0015C Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Strain: 000664 Oligonucleotides S. epidermidis psmd 1kb Upstream Forward (KpnI) 50-GTCCGGTACCCACAA ATAATGTTGCACCCC-30 This Study S. epidermidis psmd 1kb Upstream Reverse (SacII) 50-GTTCCCGCGGGTTCAT GACCTCCTTTCAAAAGG-30 This Study S. epidermidis psmd 1kb Down Forward (SalI) 50- GTCCGTCGACCGCTG AGAGTAATCATTAATTTGC-30 This Study S. epidermidis psmd 1kb Down Reverse (SacII) 50- GTCACCGCGGTTCTA CATGGGCCTGG-30 This Study S. epidermidis psmd 1kb Internal Forward 50-AGCAAGAGTGTCAA TGGTTAC-30 This Study S. epidermidis psmd 1kb Internal Reverse 50-CTAAGAAAGCGAGC CAAC-30 This Study (Continued on next page) Cell Reports 42, 113024, September 26, 2023 13 ..

    DNA Purification:

    Article Title: Staphylococcus epidermidis activates keratinocyte cytokine expression and promotes skin inflammation through the production of phenol-soluble modulins.
    Article Snippet: 1457 Dpsmε (1B 1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 Dhld (1C-1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DpsmdDhld (D5-1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DecpA (1C-1(1)) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DpsmdDhldDecpA (1D-2(1)) Gallo (UCSD) This Study E. coli Chemically Competent One Shot Top 10 Invitrogen Cat#C404010 E. coli (DC10B) Monk and Foster18 N/A Chemicals, peptides, and recombinant proteins 2-mercaptoethanol Sigma-Aldrich Cat# M6250 Antibiotic-Antimycotic (1003) Gibco Cat# 15240062 Bacto Agar BD Biosciences Cat# 214010 Calcium chloride dihydrate Sigma-Aldrich Cat# 22,350-6 Defined Trypsin Inhibitor (DTI) Gibco Cat# R-007-100 DPBS Gibco Cat# 14190-144 Human keratinocyte growth supplement (HKGS) Gibco Cat# S0015 EpiLife complete medium, with 60 M calcium Gibco Cat# MEPI500CA RPMI medium Gibco Cat#11875093 Liberase TL Roche Cat# 5401020001 DNAse Sigma Cat#D5025 Fetal Bovine Serum Corning Cat#35010CV Ethyl alcohol, Pure Sigma-Aldrich Cat# E7023 Formalin Azer Scientific Cat# PFNBF-20 Lysing Matrix B MP Biomedical Cat# 116911050-CF Lysing Matrix D MP Biomedical Cat# 116913050-CF Molecular biology grade TE buffer Invitrogen Cat# AM9849 (Continued on next page) 12 Cell Reports 42, 113024, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAlater Stabilization Solution Invitrogen Cat# AM7021 Tryptic soy Broth (TSB) Sigma-Aldrich Cat# T8907-1KG Trypsin/EDTA solution Gibco Cat# R-001-100 UltraPure distilled water Invitrogen Cat# 10977-015 Q5 Hot Start High Fidelity Polymerase Master Mix (2x) NEB Cat#M0494L Ultrapure Agarose Thermofisher Scientific Cat# 16500500 1kb Plus DNA Ladder NEB Cat#N3200L Sybr Safe DNA Gel Stain Thermofisher Scientific Cat#S33102 SYBR Green qPCR Master Mix (23) Biomiga Cat# QP1311-02 Prolong Antifade Gold Thermofisher Scientific Cat# P36934 S. epidermidis PSMd, fMSIVSTIIEVVKTIVDIVKKFKK Lifetein N/A S. epidermidis PSMa, fMADVIAKIVEIVKGLIDQFTQK Lifetein N/A S. epidermidis PSMe, fMFIINL VKKVISFIKGLFGNNENE Lifetein N/A S. epidermidis d-toxin (hld), fMAADIISTIGDLVKWIIDTVNKFKK Lifetein N/A Optima LC/MS Water Fisher Scientific CAS 7732-18-5 Optima LC/MS Acetonitrile Fisher Scientific CAS 75-05-8 Optima LC/MS Formic Acid Fisher Scientific CAS 64-18-6 Optima LC/M Methanol Fisher Scientific CAS 67-56-1 Critical commercial assays iSCRIPT cDNA synthesis Kit BIO-RAD Cat#1708891 PureLink RNA Mini Kit Invitrogen Cat# 12183025 PureLink Microbiome DNA Purification Kit Invitrogen Cat# A29790 Human IL-8/CXCL8 DuoSet ELISA R&D Systems Cat# DY208 Deposited data Human keratinocyte S. epidermidis PSM treatment RNA-seq (bulk) This Study GEO: GSE209653 Murine S. epidermidis PSM treatment RNA-seq (bulk) This Study GEO: GSE210046 Experimental models: Cell lines Human Epidermal Keratinocytes, neonatal (HEKn) Gibco Cat# C0015C Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Strain: 000664 Oligonucleotides S. epidermidis psmd 1kb Upstream Forward (KpnI) 50-GTCCGGTACCCACAA ATAATGTTGCACCCC-30 This Study S. epidermidis psmd 1kb Upstream Reverse (SacII) 50-GTTCCCGCGGGTTCAT GACCTCCTTTCAAAAGG-30 This Study S. epidermidis psmd 1kb Down Forward (SalI) 50- GTCCGTCGACCGCTG AGAGTAATCATTAATTTGC-30 This Study S. epidermidis psmd 1kb Down Reverse (SacII) 50- GTCACCGCGGTTCTA CATGGGCCTGG-30 This Study S. epidermidis psmd 1kb Internal Forward 50-AGCAAGAGTGTCAA TGGTTAC-30 This Study S. epidermidis psmd 1kb Internal Reverse 50-CTAAGAAAGCGAGC CAAC-30 This Study (Continued on next page) Cell Reports 42, 113024, September 26, 2023 13 ..

    RNA Sequencing:

    Article Title: Staphylococcus epidermidis activates keratinocyte cytokine expression and promotes skin inflammation through the production of phenol-soluble modulins.
    Article Snippet: 1457 Dpsmε (1B 1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 Dhld (1C-1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DpsmdDhld (D5-1) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DecpA (1C-1(1)) Gallo (UCSD) This Study Staphylococcus epidermidis 1457 DpsmdDhldDecpA (1D-2(1)) Gallo (UCSD) This Study E. coli Chemically Competent One Shot Top 10 Invitrogen Cat#C404010 E. coli (DC10B) Monk and Foster18 N/A Chemicals, peptides, and recombinant proteins 2-mercaptoethanol Sigma-Aldrich Cat# M6250 Antibiotic-Antimycotic (1003) Gibco Cat# 15240062 Bacto Agar BD Biosciences Cat# 214010 Calcium chloride dihydrate Sigma-Aldrich Cat# 22,350-6 Defined Trypsin Inhibitor (DTI) Gibco Cat# R-007-100 DPBS Gibco Cat# 14190-144 Human keratinocyte growth supplement (HKGS) Gibco Cat# S0015 EpiLife complete medium, with 60 M calcium Gibco Cat# MEPI500CA RPMI medium Gibco Cat#11875093 Liberase TL Roche Cat# 5401020001 DNAse Sigma Cat#D5025 Fetal Bovine Serum Corning Cat#35010CV Ethyl alcohol, Pure Sigma-Aldrich Cat# E7023 Formalin Azer Scientific Cat# PFNBF-20 Lysing Matrix B MP Biomedical Cat# 116911050-CF Lysing Matrix D MP Biomedical Cat# 116913050-CF Molecular biology grade TE buffer Invitrogen Cat# AM9849 (Continued on next page) 12 Cell Reports 42, 113024, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAlater Stabilization Solution Invitrogen Cat# AM7021 Tryptic soy Broth (TSB) Sigma-Aldrich Cat# T8907-1KG Trypsin/EDTA solution Gibco Cat# R-001-100 UltraPure distilled water Invitrogen Cat# 10977-015 Q5 Hot Start High Fidelity Polymerase Master Mix (2x) NEB Cat#M0494L Ultrapure Agarose Thermofisher Scientific Cat# 16500500 1kb Plus DNA Ladder NEB Cat#N3200L Sybr Safe DNA Gel Stain Thermofisher Scientific Cat#S33102 SYBR Green qPCR Master Mix (23) Biomiga Cat# QP1311-02 Prolong Antifade Gold Thermofisher Scientific Cat# P36934 S. epidermidis PSMd, fMSIVSTIIEVVKTIVDIVKKFKK Lifetein N/A S. epidermidis PSMa, fMADVIAKIVEIVKGLIDQFTQK Lifetein N/A S. epidermidis PSMe, fMFIINL VKKVISFIKGLFGNNENE Lifetein N/A S. epidermidis d-toxin (hld), fMAADIISTIGDLVKWIIDTVNKFKK Lifetein N/A Optima LC/MS Water Fisher Scientific CAS 7732-18-5 Optima LC/MS Acetonitrile Fisher Scientific CAS 75-05-8 Optima LC/MS Formic Acid Fisher Scientific CAS 64-18-6 Optima LC/M Methanol Fisher Scientific CAS 67-56-1 Critical commercial assays iSCRIPT cDNA synthesis Kit BIO-RAD Cat#1708891 PureLink RNA Mini Kit Invitrogen Cat# 12183025 PureLink Microbiome DNA Purification Kit Invitrogen Cat# A29790 Human IL-8/CXCL8 DuoSet ELISA R&D Systems Cat# DY208 Deposited data Human keratinocyte S. epidermidis PSM treatment RNA-seq (bulk) This Study GEO: GSE209653 Murine S. epidermidis PSM treatment RNA-seq (bulk) This Study GEO: GSE210046 Experimental models: Cell lines Human Epidermal Keratinocytes, neonatal (HEKn) Gibco Cat# C0015C Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Strain: 000664 Oligonucleotides S. epidermidis psmd 1kb Upstream Forward (KpnI) 50-GTCCGGTACCCACAA ATAATGTTGCACCCC-30 This Study S. epidermidis psmd 1kb Upstream Reverse (SacII) 50-GTTCCCGCGGGTTCAT GACCTCCTTTCAAAAGG-30 This Study S. epidermidis psmd 1kb Down Forward (SalI) 50- GTCCGTCGACCGCTG AGAGTAATCATTAATTTGC-30 This Study S. epidermidis psmd 1kb Down Reverse (SacII) 50- GTCACCGCGGTTCTA CATGGGCCTGG-30 This Study S. epidermidis psmd 1kb Internal Forward 50-AGCAAGAGTGTCAA TGGTTAC-30 This Study S. epidermidis psmd 1kb Internal Reverse 50-CTAAGAAAGCGAGC CAAC-30 This Study (Continued on next page) Cell Reports 42, 113024, September 26, 2023 13 ..



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    Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, IL-8, and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, IL-8, and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Article Snippet: For the samples of the ex vivo whole blood model, the plasma concentrations of matrix metallopeptidase 9 (MMP9, #DY911, R&D Systems, Minneapolis, USA), IL-6 (#555220, BD Biosciences, San Jose, USA), and IL-8 (#DY208, R&D Systems) were measured in citrate-anticoagulated plasma using enzyme-linked immunosorbent assay according to the respective manufacturer’s instructions.

    Techniques: Concentration Assay, Incubation, Ex Vivo, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Bacteria

    Diagnostic performance for the detection of bacteremia, analyzing the neutrophil phenotype by determining the median fluorescence intensity (MFI) and the cellular response capacity (CRC) in comparison with traditional markers of humoral inflammation (IL-6, IL-8, MMP9). a Comparison of receiver operating characteristic (ROC) at 10,000 CFU/ml Escherichia coli ( E. coli ) with the respective 95% confidence interval (CI) and P -value, and half-maximal effective concentration (EC 50 ) as a function of the E. coli concentration. b Detailed comparison of the EC 50 as a function of the E. coli concentration. c Exemplary comparison of EC 50 curve fit after normalization as indicated by EC 50 (%) on the respective Y-axis to BuC=100% and 50 000 CFU/ml E. coli =0% for the humoral marker IL-6 (the IL-6 values were multiplied by −1 before EC 50 calculation to facilitate comparability with the CRC) and the change in neutrophil phenotype represented by CD11b CRC. BuC indicates buffer control after 60 min incubation; numbers on the X-axis of c indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, evaluating the EC 50 of IL-8, MMP9, the MFI, and CRC of CD10, CD11b, and CD62L in comparison to the EC 50 of IL-6. P -values are indicated above the respective data points. ⁎ P <0.05. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Diagnostic performance for the detection of bacteremia, analyzing the neutrophil phenotype by determining the median fluorescence intensity (MFI) and the cellular response capacity (CRC) in comparison with traditional markers of humoral inflammation (IL-6, IL-8, MMP9). a Comparison of receiver operating characteristic (ROC) at 10,000 CFU/ml Escherichia coli ( E. coli ) with the respective 95% confidence interval (CI) and P -value, and half-maximal effective concentration (EC 50 ) as a function of the E. coli concentration. b Detailed comparison of the EC 50 as a function of the E. coli concentration. c Exemplary comparison of EC 50 curve fit after normalization as indicated by EC 50 (%) on the respective Y-axis to BuC=100% and 50 000 CFU/ml E. coli =0% for the humoral marker IL-6 (the IL-6 values were multiplied by −1 before EC 50 calculation to facilitate comparability with the CRC) and the change in neutrophil phenotype represented by CD11b CRC. BuC indicates buffer control after 60 min incubation; numbers on the X-axis of c indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, evaluating the EC 50 of IL-8, MMP9, the MFI, and CRC of CD10, CD11b, and CD62L in comparison to the EC 50 of IL-6. P -values are indicated above the respective data points. ⁎ P <0.05. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Article Snippet: For the samples of the ex vivo whole blood model, the plasma concentrations of matrix metallopeptidase 9 (MMP9, #DY911, R&D Systems, Minneapolis, USA), IL-6 (#555220, BD Biosciences, San Jose, USA), and IL-8 (#DY208, R&D Systems) were measured in citrate-anticoagulated plasma using enzyme-linked immunosorbent assay according to the respective manufacturer’s instructions.

    Techniques: Diagnostic Assay, Fluorescence, Comparison, Concentration Assay, Marker, Control, Incubation, Bacteria

    Clinical specifications and parameters over all time points of the sepsis cohort. a Suspected infection cause of sepsis. b Distribution of the individual score points of the Sequential Organ Failure Assessment (SOFA) score. c Total SOFA score. d-h Traditional and humoral markers of inflammation: leukocytes and neutrophil-lymphocyte ratio ( d ), C-reactive protein (CRP) and procalcitonin (PCT) ( e ), interleukin-6 (IL-6) and interleukin-8 (IL-8) ( f ), serum amyloid A (SAA) and calprotectin ( g ), matrix metallopeptidase 9 (MMP9) and myeloperoxidase (MPO) ( h ). Values are shown as median and interquartile range. n =14. CNS. Central nervous system; HV. Healthy volunteers.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Clinical specifications and parameters over all time points of the sepsis cohort. a Suspected infection cause of sepsis. b Distribution of the individual score points of the Sequential Organ Failure Assessment (SOFA) score. c Total SOFA score. d-h Traditional and humoral markers of inflammation: leukocytes and neutrophil-lymphocyte ratio ( d ), C-reactive protein (CRP) and procalcitonin (PCT) ( e ), interleukin-6 (IL-6) and interleukin-8 (IL-8) ( f ), serum amyloid A (SAA) and calprotectin ( g ), matrix metallopeptidase 9 (MMP9) and myeloperoxidase (MPO) ( h ). Values are shown as median and interquartile range. n =14. CNS. Central nervous system; HV. Healthy volunteers.

    Article Snippet: For the samples of the ex vivo whole blood model, the plasma concentrations of matrix metallopeptidase 9 (MMP9, #DY911, R&D Systems, Minneapolis, USA), IL-6 (#555220, BD Biosciences, San Jose, USA), and IL-8 (#DY208, R&D Systems) were measured in citrate-anticoagulated plasma using enzyme-linked immunosorbent assay according to the respective manufacturer’s instructions.

    Techniques: Infection